Yes you can. I have an OCZ Vertex 2 60GB SSD with a 1TB Sata HDD with another 1TB as a backup. The SSD contains operationg system plus most of my programs. My games (Steam)are on the !TB drive as is all my photos,videos and music. After installing the OS on the SSD move all the MY folders location to the bigger drive, a very simple process, this will mean that if you add music photo etc they will automatically go to the bigger drive.
iMac Pro review