RAM - The price of RAM is said to be very low at the moment. Remove all the old RAM and replace it with the most that your Lappy will hold. (Buy from Crucial, they will sort you out if there are any problems) I don't think that it matters much if that is made up of 1 Stick or 2.
TuneUp is outside my experience as I use CCleaner (free) and the Built-in Windows Defragmenter. I also use Secunia (free) to warn me if any of my Software is un-patched.